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Tissue disposition of benzo[ A ]pyrene in blue mussel ( Mytilus edulis ) and effect of algal concentration on metabolism and depuration
Author(s) -
Magnusson Kerstin,
Ekelund Rolf,
Ingebrigtsen Kristian,
Granmo Åke,
Brandt Ingvar
Publication year - 2000
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620191111
Subject(s) - mytilus , isochrysis galbana , benzo(a)pyrene , blue mussel , mussel , pyrene , hepatopancreas , bioaccumulation , metabolism , gill , metabolite , chemistry , clearance rate , biology , microsome , environmental chemistry , bivalvia , bioconcentration , biochemistry , mollusca , algae , botany , in vitro , ecology , fishery , endocrinology , organic chemistry , fish <actinopterygii>
Abstract In vivo metabolism and depuration of benzo [ a ]pyrene (B a P) was studied in the blue mussel Mytilus edulis . In one experiment, the animals were injected with 3 H‐B a P and thereafter exposed to either a high (H) or a low (L) concentration of the alga Isochrysis galbana . Mussels were sampled after 2, 4, 8, 16, and 32 d and temporal changes in the metabolite profiles were analyzed using nonpolar and polar solvents. The metabolic fate and the depuration rate of B a P from the two feeding groups were compared. In a second experiment, mussels were exposed to [ 3 H]‐B a P or [ 14 C]‐B a P either injected or via the surrounding water, and the tissue distribution of radiolabeled compound was studied. The half‐life of B a P was 15–17 days and unaffected by the food concentration. The metabolic profile differed slightly between the two treatments. Animals in the L group had a significant peak in the quantity of macromolecular adducts eight days after the injection, whereas the H group had a fairly stable amount of adducts throughout the experimental period. The tissue distribution study revealed that the radiolabeled compound was mainly present in the epithelial cells of the hepatopancreas and the ciliated epithelial cells of the gills. A fraction of the radioactivity was firmly bound.