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Bioavailability of polycyclic aromatic hydrocarbons and polychlorinated biphenyls in rats from naturally contaminated soils—preliminary evaluation of the influence of soil parameters
Author(s) -
Billeret Mikakline,
Berny Philippe,
Mazallon Michelle,
Buronfosse Thierry
Publication year - 2000
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620191030
Subject(s) - bioavailability , environmental chemistry , chemistry , pollutant , soil water , organic matter , persistent organic pollutant , soil contamination , ecotoxicology , environmental science , organic chemistry , soil science , biology , bioinformatics
In order to assess the bioavailability of organic pollutants (polycyclic aromatic hydrocarbons [PAHs],polychlorobiphenyls [PCBs]) in polluted soils, male laboratory rats were exposed during 3 d and one night to two types of soil. These soils were highly polluted by PAHs (F 2 soil) and by PCBs (S 3 soil). They were diluted (1:1) either with an organic or a mineral matrix. Those matrices had different characteristics, and the purpose was to determine the influence of several soil parameters (organic matter) on the pollutants' bioavailability. Two categories of biomarkers were used: pollutant's burden and activities of microsomal liver and lung's cytochrome P450 1A‐monooxygenase, namely, ethoxyresorufin‐ O ‐deethylase (EROD) activities. Absorption differed between exposed rats, depending on the pollutant and on the organ considered. Their EROD activity was well correlated with PAH and PCB burden, respectively. Moreover, EROD activity and pollutants burden in both organs depended on the dilution performed. The PAH bioavailability was lower after a dilution with organic matrices. In fact, those matrices had both the highest organic matter concentration and the lowest granulometry and appeared to adsorb more. Thus, organic pollutant bioavailability varies according to the soil characteristics. The most important parameters seemed to be organic matter and granulometry. The EROD activity and CYP1A1 dosage were good biomarkers and closely monitored the differences in bioavailability.