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Exposure to lead induces hypoxia—like responses in bullfrog larvae (Rana catesbeiana)
Author(s) -
Rice Timothy M.,
Blackstone Brian J.,
Nixdorf Wendy L.,
Taylor Douglas H.
Publication year - 1999
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620181024
Subject(s) - bullfrog , rana , larva , hypoxia (environmental) , biology , zoology , chemistry , oxygen , anatomy , ecology , organic chemistry
Amphibians collected around mining sites, areas with extensive automobile traffic, and shooting ranges have been documented to contain high levels of lead. Lead—exposed amphibians might respond as if in hypoxic conditions because exposure is known to decrease hemoglobin levels, damage erythrocytes, and alter respiratory surfaces. Therefore, we exposed bullfrog larvae to either 0 or 780 μg/L Pb and either 3.50 or 7.85 mg/L oxygen for 7 d and monitored activity, trips to the surface, and buccal ventilation rates. Activity was significantly decreased in larvae exposed to low oxygen, Pb, or both compared to activity of larvae in high oxygen with no Pb. Larvae exposed to both Pb and low oxygen displayed higher buccal ventilation rates than larvae exposed to either treatment separately. Lead—exposed larvae surfaced significantly more often than unexposed larvae even under high—oxygen conditions. Lead—exposed larvae decreased in mass during the exposure period, whereas unexposed larvae increased in mass. Lead exposure could decrease survival of larvae in the field not only because of physiological problems due to decreased oxygen uptake but also because of greater predation pressure due to increased presence at the surface and reduced growth rates.