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Copper binding affinity of rainbow trout ( Oncorhynchus mykiss ) and brook trout ( Salvelinus fontinalis ) gills: Implications for assessing bioavailable metal
Author(s) -
MacRae Russell K.,
Smith Darren E.,
SwobodaColberg Norbert,
Meyer Joeseph S.,
Bergman Harold L.
Publication year - 1999
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620180616
Subject(s) - salvelinus , rainbow trout , copper , gill , environmental chemistry , trout , fontinalis , bioavailability , bioassay , biotic ligand model , copper toxicity , chemistry , toxicity , genetic algorithm , biology , ecotoxicology , ecology , fishery , fish <actinopterygii> , organic chemistry , bioinformatics
In this study, we determined the conditional stability constant (log K ′) of copper for the gills of rainbow trout ( Oncorhynchus mykiss; RBT) and brook trout ( Salvelinus fontinalis; BT). Using toxicity‐based complexation bioassays, which measure the effect of competing organic ligands on copper toxicity, the RBT gill copper log K ′ range was 6.4 to 7.2. Using a Scatchard analysis of gill Cu accumulation, the RBT log K ′ was 7.50 and the BT log K ′ was 7.25. The close agreement in RBT log K ′ values between these two methods suggests that measurement of gill copper accumulation is an acceptable alternative for determining a toxicity‐based gill copper binding affinity. Our results also suggest that there is either a single gill copper binding component or, more realistically, multiple components with similar binding properties that function collectively to define a single toxicologically relevant copper conditional stability constant. These results suggest analytical approaches to measuring bioavailable metal concentrations, such as geochemical modeling where biological ligands are included in speciation calculations, may adequately simulate complex biological ligands. A method to convert gill copper accumulation to a bioavailable water criterion is also discussed.