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Volatile fractions of landfill leachates and their effect on Chlamydomonas reinhardtii : In vivo chlorophyll a fluorescence
Author(s) -
Brack Werner,
Rottler Horst,
Frank Hartmut
Publication year - 1998
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620171014
Subject(s) - leachate , chlamydomonas reinhardtii , bioassay , environmental chemistry , chemistry , chlamydomonas , toxicity , hydrogen sulfide , chlorophyll , biology , organic chemistry , biochemistry , ecology , sulfur , mutant , gene
A method for identification of toxic volatile constituents of landfill leachates is presented that combines bioassay‐compatible sample preparation, chemical analysis, and a bioassay based on in vivo chlorophyll a fluorescence of the green alga Chlamydomonas reinhardtii. Two major pathways of toxicity were identified by comparing fluorescence patterns: specific toxicity of hydrogen sulfide, and narcotic action of nonreactive organic compounds. For quantification, the contributions of identified compounds were calculated using toxic units. The ecotoxicologic relevance of volatile fractions from hazardous waste leachates was shown.