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Promutagen activation by the green alga Selenastrum capricornutum
Author(s) -
Sauser Kathleen R.,
Chung KingThom,
Wong TitYee
Publication year - 1998
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620170923
Subject(s) - selenastrum , algae , chlorophyta , biochemistry , peroxidase , chlorophyceae , chlorella , chemistry , green algae , cytochrome p450 , biology , botany , enzyme
The capability of Selenastrum capricornutum to activate o ‐phenylenediamine (OPD) and 2‐aminofluorene (2‐AF) was tested in a modified Ames assay in which the activation component (S9) was replaced by the algal cells. Normal‐grown algae did not activate OPD but these cells activated 2‐AF by sixfold. Copper induces a peroxidase in S. capricornutum . Algae pregrown on copper‐enriched medium increased the mutagenicity of OPD by more than 16‐fold, but these cells failed to further activate 2‐AF. However, 2‐AF was further activated by algae pretreated with flavone, Aroclor, 5,6‐naphthoflavone, or 2,4‐dichlorophenoxyacetic acid, but not lindane or ethanol. This suggested that a cytochrome P450 system was involved in 2‐AF activation. Coincubating algae with cytochrome P450 inhibitors including diethyldithiocarbamate, methimizole, or acetaminophen, but not catechin, 7–8‐naphthoflavone, or CuCl 2 reduced the activation level of 2‐AF significantly.