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Bioassay technique using nonspecific esterase activities of tetrahymena pyriformis for screening and assessing cytotoxicity of xenobiotics
Author(s) -
Bogaerts Philippe,
Senaud Jean,
Bohatier Jacques
Publication year - 1998
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620170822
Subject(s) - tetrahymena pyriformis , bioassay , daphnia magna , fluorescein , xenobiotic , chemistry , toxicity , cytotoxicity , chromatography , esterase , chemical compound , ecotoxicity , sodium dodecyl sulfate , ec50 , tetrahymena , biochemistry , biology , toxicology , environmental chemistry , fluorescence , enzyme , in vitro , organic chemistry , ecology , physics , quantum mechanics
A simple and rapid test for screening and assessing the cytotoxicity of xenobiotics was developed with Tetrahymena pyriformis. The method estimates the activities of nonspecific esterases of a cell by concentrating within it a specific amount of fluorescence associated with fluorescein dye. The 1‐h median effective concentration (EC50) values of 10 inorganic and eight organic substances are presented and compared to those of three other bioassays: the conventional T. pyriformis proliferation rate 9‐h median inhibitory concentrations, the Microtox® 30‐min EC50s, and the Daphnia magna 4‐methylumbelliferyl β‐D galactoside 1‐h EC50s. A highly significant correlation was found between the results obtained with the fluorescein diacetate test and those obtained with the growth inhibition and Microtox tests. This in vivo enzymatic test showed high sensitivity to all compounds tested except Cr 6+ and sodium dodecyl sulfate.