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Responses of carp hepatopancreatic 7‐ethoxyresorufin‐ O ‐deethylase and glutathione‐dependent enzymes to organic pollutants—a field study
Author(s) -
Machala Miroslav,
Petřivalský Marek,
Nezveda Karel,
Ulrich Robert,
Dušek Ladislav,
Piačka Vladimír,
Svobodová Zdenka
Publication year - 1997
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620160713
Subject(s) - glutathione , carp , chemistry , biomonitoring , pollutant , environmental chemistry , microsome , glutathione reductase , glutathione s transferase , cytochrome p450 , enzyme , biochemistry , biology , glutathione peroxidase , fishery , organic chemistry , fish <actinopterygii>
Modulations of hepatopancreatic activities of cytochrome P4501A (CYP1A) and glutathione‐dependent enzymes were investigated in carp collected in five ponds with different levels of contamination. The CYP1A‐dependent 7‐ethoxyresorufin‐ O ‐deethylase activity was markedly induced by polycyclic aromatic hydrocarbons present in the sediment at a total concentration of 0.9 mg/kg. Even a low organic contamination increased some of the glutathione‐dependent enzymatic activities, namely cytosolic glutathione reductase, glutathione S ‐transferase toward 1‐chloro‐2,4‐dinitrobenzene, ethacrynic acid and l,2‐epoxy‐3‐(p‐nitrophenoxy)propane, and microsomal glutathione S ‐transferase. These parameters should be considered as potential tools for the biomonitoring of exposure to chemicals and/or impacts of exposure. An example of a multivariate cluster and discriminant analysis of the obtained analytical and biochemical data proved to be very effectiv tools for the characterization of the level of contamination.