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Effects of sublethal copper exposure on muscle energy metabolism of common carp, measured by 31 P‐nuclear magnetic resonance spectroscopy
Author(s) -
De Boeck Gudrun,
Borger Robert,
Van der Linden Annemie,
Blust Ronny
Publication year - 1997
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620160411
Subject(s) - phosphocreatine , cyprinus , copper , chemistry , in vivo , intracellular , intracellular ph , medicine , common carp , carp , metabolism , high energy phosphate , nuclear magnetic resonance spectroscopy , energy metabolism , adenosine triphosphate , zoology , endocrinology , radiochemistry , biochemistry , biology , fish <actinopterygii> , microbiology and biotechnology , organic chemistry , fishery
The effects of shock and subchronic exposure(1 week) to 0.36 ± 0.08 and 1.31 ± 0.22 μM of copper on the energy metabolism of common carp ( Cyprinus carpio ) were studied by means of in vivo 31 P‐nuclear magnetic resonance spectroscopy ( 31 P‐NMRS). During the experiments, fish were submitted to an additional hypoxic challenge and recovery from this challenge was followed for 6 hs. During all experiments adenosine triphosphate (ATP) levels remained stable. Under control conditions, levels of phosphocreatine (P Cr ) and inorganic phosphate (P i ) recovered rapidly after the hypoxic challenge; however, full recovery was not observed after shock copper exposure. Also, intracellular pH (pH i ) did not recover from the hypoxic challenge after shock exposure. After 1 week of exposure the fish had clearly developed an increased tolerance to copper. At both copper concentrations, P Cr and P i levels returned to resting levels after the hypoxic challenge, but at the highest copper concentration P Cr to P i ratios were significantly lower than P Cr to P i ratios in the control group and levels of P Cr and P i were very unstable. At the high copper concentration, pHi was clearly decreased compared to the control group even before the hypoxic challenge.