Premium
Influence of pH on the toxic effects of zinc, cadmium, and pentachlorophenol on pure cultures of soil microorganisms
Author(s) -
van Beelen Patrick,
FleurenKemilä Arja K.
Publication year - 1997
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620160208
Subject(s) - pentachlorophenol , chemistry , cadmium , pseudomonas putida , zinc , environmental chemistry , aspergillus niger , bioremediation , ec50 , zinc toxicity , microorganism , sorption , soil contamination , food science , soil water , bacteria , biochemistry , contamination , ecology , biology , organic chemistry , genetics , adsorption , in vitro , enzyme
In this study the effect of the acidification of soil pore water on the uptake and toxicity of cationic and anionic pollutants was measured in an experimental model system. The influence of pH on the toxic effects of zinc, cadmium, and pentachlorophenol was studied in buffered suspensions of pure cultures of soil microorganisms. In this system the speciation of the toxicant, the pH, and the biomass are defined, constant, and thus easier to study than in a system with the solid soil matrix and pore water. The mineralization of [ 14 C]acetate to 14 CO 2 was used to measure the toxic effects of pollutants on a fungus ( Aspergillus niger CBS 121.49), an actinomycete ( Streptomyces lividans 66), two Gram‐negative Pseudomonas putida strains (MT‐2 and DSM 50026), and a Gram‐positive strain ( Rhodococcus erythropolis A177). Large differences in sensitivity were observed between the species. For pentachlorophenol the highest EC50 was 81 mg/L for Pseudomonas putida at pH 8, whereas the lowest was 0.13 mg/L for Aspergillus niger at pH 6. Aspergillus niger was not sensitive to 1,000 mg Zn/L, whereas Pseudomonas putida at pH 7.8 showed the lowest EC50, 0.14 mg Zn/L. When pH was increased, pentachlorophenol became less toxic and showed less sorption to the biomass, whereas zinc and cadmium became more toxic and showed more sorption to the biomass. The results indicate that higher pore‐water concentrations due to acidification of zinc‐ and cadmium‐polluted soils may not be accompanied by increased toxic effects on microorganisms because of the relatively low toxicity of these metals in pore water at low pH.