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Lyophilization of thylakoids for improved handling in a bioassay
Author(s) -
Zimmermann Gitta M.,
Kramer Gerard N.,
Schnabl Heide
Publication year - 1996
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620150905
Subject(s) - bioassay , thylakoid , isolation (microbiology) , photosynthesis , biology , chromatography , chemistry , biochemistry , chloroplast , ecology , microbiology and biotechnology , gene
The thylakoid bioassay is used to determine the effect of herbicides in water. To avoid isolation for each bioassay, lyophilization of thylakoids for continuous, reproducible supply as bioassay material is descibed. This technique maintains the photosynthetic activity after lyophilization at 82 ± 9% ( n = 5) of its original level.