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Phytotoxicity, uptake and metabolism of 1,4‐dichlorobenzene by plant cells
Author(s) -
Jones Kevin C.,
Wang MinJian,
Bokern Maria,
Boehme Christian,
Harms Hans
Publication year - 1996
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620150714
Subject(s) - daucus carota , phytotoxicity , lycopersicon , metabolism , glycine , biology , botany , dichlorobenzene , horticulture , chemistry , food science , biochemistry , amino acid , organic chemistry
Phytotoxicity, uptake, and metabolism of 14‐dichlorobenzene (1,4‐DCB) by carrot ( Daucus carota L.), soybean ( Glycine max. L.), tomato (Lycopersicon esculentum Mill.), and red goosefoot (Chenopodiun rubrum L.) cell suspension cultures were studied. Sealed glass systems were utilized for the investigation because 1,4‐DCB is volatile. The sealed systems affect the growth of plant cells, but do not provide different results when testing xenobiotic uptake and metabolism. 1,4‐Dichlorobenzene (40 μg in 40 ml medium) was taken up by carrot (49%), soybean (50%), and red goosefoot (62%) cells. Only the soybean cell cultures provided evidence of the existence of metabolites of this compound, probably conjugates of chlorophenols. Conditions for phytotoxicity tests were modified because the growth of cell cultures was affected when sealed for longer than 2 d. 1,4‐Dichlorobenzene is toxic to cell cultures of the three tested plant species (tomato, soybean, and carrot). Concentrations of 0.5 mM caused 50% growth inhibition in carrot and soybean cultures. The tomato cultures were more sensitive, with 0.05 mM causing 50% growth inhibition.