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Comparison of the response of bacterial luminescence and mitochondrial respiration to the effluent of an oil refinery
Author(s) -
Riisberg Marianne,
Bratlie Ellen,
Stenersen Jørgen
Publication year - 1996
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620150414
Subject(s) - photobacterium phosphoreum , luminescent bacteria , effluent , respiration , refinery , ec50 , environmental chemistry , chemistry , clark electrode , biology , toxicity , biochemistry , environmental science , environmental engineering , botany , electrode , organic chemistry , electrolyte , in vitro
The effects of oil refinery effluents on rat mitochondrial respiration and on the luminescence of the bacterium Photo‐bacterium phosphoreum were compared. Mitochondria from male Wistar rat livers were exposed to different concentrations of refinery effluents in a semiclosed 3–ml reaction vessel. Respiration was measured polarographically with an oxygen electrode. Effects on P. phosphoreum were measured by the standard test developed by Microbics. The mitochondrial method showed EC50s in the range from 1 to 7.5%, while Microtox gave EC50 in the range from 30 to 42%. The higher sensitivity of mitochondria may be exploited in the development of a sensitive biosensor for toxicity of oil refinery effluents.