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Ceriofast™: An acute toxicity test based on Ceriodaphnia dubia feeding behavior
Author(s) -
Bitton Gabriel,
Rhodes Kimberly,
Koopman Ben
Publication year - 1996
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620150208
Subject(s) - ceriodaphnia dubia , bioassay , pentachlorophenol , toxicity , toxicant , environmental chemistry , acute toxicity , toxicology , brachionus calyciflorus , biology , chemistry , ecology , rotifer , organic chemistry
We have developed a rapid acute toxicity test (CerioFAST™) based on suppression of feeding activity of Ceriodaphnia dubia in the presence of toxicants. The bioassay consists of a 1‐h exposure period to a given toxicant. Yeast cells, stained with a fluorescent dye, are added 20 min before the end of the exposure period. Response to a toxic sample is indicated by the absence of fluorescence in the gut of the daphnids. CerioFAST was compared to the standard 48‐h C. dubia acute bioassay, using heavy metals and organic compounds. CerioFAST EC50s of Cd, Cu, Pb, Ag, Zn, and carbofuran were in the 0.01–0.1‐mg/L range, whereas EC50s of hexachloroethane, pentachlorophenol, trichlorophenol, and lindane were in the 1–10‐mg/L range. CerioFAST EC50s of the heavy metals and organics were well correlated with EC50s obtained with the 48‐h C. dubia bioassay.