Premium
Metabolism of different PCB congeners in plant cell cultures
Author(s) -
Wilken Annelen,
Bock Claudia,
Bokern Maria,
Harms Hans
Publication year - 1995
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620141203
Subject(s) - metabolism , hydrolysis , metabolite , perchloric acid , biochemistry , chemistry , biology , environmental chemistry , organic chemistry
The metabolism of 10 different congeners of polychlorinated biphenyls (PCBs) was tested in cell cultures of 12 different plant species. The rate of metabolism was determined through comparison with cells inactivated by treatment with perchloric acid. The metabolism of defined PCB congeners was strongly dependent on the plant species. In particular, two Fabaceae species exhibited high capacities for metabolizing different congeners. Except for PCB 15 (4,4′‐dichlorobiphenyl), it was shown that a lower chlorination grade is associated with higher metabolism rates. Also, it was shown that substitution and molecular configuration play an important role in the metabolism of PCBs in plants. In studies with 14 C‐PCB 1 (2‐chlorobiphenyl) in soybean cultures, one dihydroxylated and six different monohydroxylated compounds were detected after acid hydrolysis of polar metabolites (conjugates). Hydrolysis of metabolites of 14 C‐PCB 52 (2,2′,5,5′‐tetrachlorobiphenyl) in wheat cell cultures yielded four monohydroxylated and three dihydroxylated metabolites.