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A method for partitioning cadmium bioaccumulated in small aquatic organisms
Author(s) -
Siriwardena P.P.G. Sunil N.,
Rana Krishen J.,
Baird Donald J.
Publication year - 1995
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620140917
Subject(s) - cadmium , ethylenediaminetetraacetic acid , oreochromis , tilapia , distilled water , bioaccumulation , chemistry , fish <actinopterygii> , environmental chemistry , zoology , chelation , chromatography , biology , fishery , organic chemistry
A series of laboratory experiments was conducted to evaluate bioaccumulation and surface adsorption of aqueous cadmium (Cd) by sac‐fry of the African tilapia Oreochromis niloticus. In the first experiment, the design consisted of two cadmium treatments: 15 μg Cd · L −1 in dilution water and a Cd‐ethylenediaminetetraacetic acid (Cd‐EDTA) complex at 15 μg · L −1 , and a water‐only control. There were five replicates per treatment and 40 fish per replicate. It was found that EDTA significantly reduced the bioaccumulation of cadmium by tilapia sac‐fry by 34%. Based on these results, a second experiment was conducted to evaluate four procedures: a no‐rinse control; rinsing in EDTA; rinsing in distilled water; and rinsing in 5% nitric acid, for removing surface‐bound Cd from exposed sac‐fry. In this experiment, 30 fish in each of five replicates were exposed to 15 μg Cd · L −1 for 72 h, processed through the rinse procedures, and analysed for total Cd. The EDTA rinse treatment significantly reduced ( p < 0.05) Cd concentrations of the exposed fish relative to those receiving no rinse. It was concluded that the EDTA rinse technique may be useful in studies evaluating the partitioning of surface‐bound and accumulated cadmium in small aquatic organisms.