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Biotransformation of di(2‐ethylhexyl)phthalate by rainbow trout
Author(s) -
Barron Mace G.,
Albro Phillip W.,
Hayton William L.
Publication year - 1995
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620140519
Subject(s) - rainbow trout , phthalate , metabolite , chemistry , biotransformation , trout , metabolism , phthalic acid , toxicokinetics , chromatography , biochemistry , biology , fish <actinopterygii> , fishery , organic chemistry , enzyme
The biotransformation of di(2‐ethylhexyl)phthalate (DEHP) was studied in rainbow trout ( Oncorhynchus mykiss ) following intravascular administration. Methyl‐esterified metabolites were identified using rodent‐derived standards and nonlinear gradient elution HPLC; metabolites were confirmed by gas chromatography. Similarities between the biotransformation of DEHP by rainbow trout and mammalian species included (a) mono‐ethylhexyl phthalate (MEHP) appeared to be the obligatory first step in DEHP metabolism; (b) the phthalate ring was not oxidized; (c) phthalic acid was a minor metabolite; and (d) several metabolites contained multiple oxidations of the 2‐ethylhexyl moiety of MEHP. No metabolites unique to rainbow trout were identified. However, fewer oxidized metabolites were identified in rainbow trout than in mammalian species, possibly due to limited mitochondrial metabolism of MEHP in rainbow trout. The amount of biliary MEHP glucuronide after intravascular administration of DEHP was substantially less than reported in rainbow trout exposed to DEHP via the water. Results confirmed that DEHP metabolism in rainbow trout proceeds by initial rapid formation of MEHP, followed by excretion or extensive oxidation by microsomal P450.