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Hepatic cytochrome P4501A induction in DAB ( Limanda limanda ) after oral dosing with the polychlorinated biphenyl mixture clophen A40
Author(s) -
Sleiderink Hedwig M.,
Everaarts Jan M.,
Goksøyr Anders,
Boon Jan P.
Publication year - 1995
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620140416
Subject(s) - limanda , polychlorinated biphenyl , chemistry , environmental chemistry , cytochrome p450 , toxicokinetics , medicine , zoology , toxicology , endocrinology , toxicity , biology , biochemistry , flatfish , fish <actinopterygii> , enzyme , fishery , organic chemistry
The flatfish dab ( Limanda limanda ) serves as an indicator species in pollution monitoring programmes in the North Sea. The present study investigated the induction response of the monooxygenase system and haematological changes in female dab after multiple administrations of a technical mixture of polychlorinated biphenyls (PCBs). Mature female dab were dosed with 1 mg of the PCB mixture Clophen A40 (Clo A40) in sunflower oil every 6 weeks, with a maximum of three doses per fish. In all PCB‐administered groups, levels of cytochrome P4501A (CYP1A) protein, measured with a semi‐quantitative ELISA method, and 7‐ethoxyresorufin O ‐deethylase (EROD) activity showed a three‐ to ninefold induction 14 d after dosing compared with control groups, smaller but also significant increases were observed in total cytochrome P450 (Σ P450) levels. Although the PCB concentrations and the corresponding toxic equivalent (TEQ) value in muscle tissue still increased after administration of the second and third dose of Clo A40, maximum responses of the EROD activity were already reached after the first dose at a TEQ value for chlorinated biphenyls (CB‐TEQ) of 2 ng/g lipid. The PCB patterns of liver and muscle tissue of female dab from the central North Sea were found to be virtually identical. Hence, the use of PCB concentrations in muscle as a qualitative model for changes in the liver appears legitimate. Haemoglobin concentrations were elevated after the third dose of Clo A40, whereas haematocrit values and the mean corpuscular haemoglobin concentration (MCHC) between treated and control groups did not differ.