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A semistatic microplate‐based phytotoxicity test
Author(s) -
Radetski Claudemir Marcos,
Ferard JeanFrançois,
Blaise Christian
Publication year - 1995
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620140215
Subject(s) - phytotoxicity , atrazine , leachate , static testing , chromatography , chemistry , effluent , environmental chemistry , pulp and paper industry , environmental science , pesticide , environmental engineering , botany , biology , ecology , engineering , structural engineering
Abstract A novel phytotoxicity test is described herein that employs a microplate equipped with membrane‐bottomed wells. This MultiScreen™ (Millipore Corp., Bedford, MA) microplate allows performance of a semistatic algal test, in which test medium is renewed periodically. With such a design, the algal test becomes comparable to other short‐term tests used to evaluate chronic toxicity of chemicals and effluents. The EC50s obtained for Cu 2+ , Cd 2+ , Cr 6+ , atrazine, and one leachate sample (municipal sludge incinerator residue) with static and semistatic algal microplate tests were compared in this study. The semistatic microplate test revealed greater sensitivity than did the static microplate test.