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A field evaluation of cytochrome P4501A as a biomarker of contaminant exposure in three species of flatfish
Author(s) -
Collier Tracy K,
Anulacion Bernadita F,
Stein John E,
Varanasi Usha,
Goksøyr Anders
Publication year - 1995
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620140116
Subject(s) - polyclonal antibodies , chemistry , immunoassay , flatfish , microsome , bioassay , cytochrome , fish <actinopterygii> , biology , environmental chemistry , ecology , antibody , biochemistry , enzyme , fishery , immunology
A study was conducted over the course of a year to determine the induction of hepatic cytochrome P4501A (CYP1A) in three species of benthic fish collected from a contaminated site compared to fish sampled from a less‐contaminated site Juvenile fish were used to minimize effects of reproductive status and migration CYP1A was determined by two catalytic assays [aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin O deethylase (EROD)] and by an immunoassay (ELISA) utilizing polyclonal antibodies raised against purified CYP1A from cod AHH activities were measured by a standard method (AHH std ) and by two variations of the standard method All three primary CYP1A measures (AHH std , EROD, and ELISA) showed consistent between site differences, indicating that induction of CYP1A can be a reliable biomarker of contaminant exposure in fish if appropriate biological variables are controlled for in field studies Multiple ANOVA demonstrated that the AHH std and ELISA data showed less variability due to species or temporal differences, and less unexplained variability, compared to the data from the EROD assay or either variation of the AHH assay For all measures, variability associated with site differences far outweighed species or temporal variability Immunoassay, while less sensitive than the AHH std assay, is nonetheless recommended to be used in conjunction with catalytic assays because of the potential for samples to lose catalytic activity if not handled properly The current results suggest that the lower noncontaminant related variability of AHH std makes this CYP1A measure potentially more useful for monitoring programs in which analysis of trends is a primary goal