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Toxicokinetics and toxicity of sediment‐associated pyrene and phenanthrene in Diporeia spp.: Examination of equilibrium‐partitioning theory and residue‐based effects for assessing hazard
Author(s) -
Landrum Peter F.,
Dupuis Wendy S.,
Kukkonen Jussi
Publication year - 1994
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620131108
Subject(s) - pyrene , phenanthrene , environmental chemistry , toxicokinetics , chemistry , sediment , toxicity , benzopyrene , benzo(a)pyrene , toxicology , biology , organic chemistry , paleontology
The amphipod Diporeia spp. was exposed to pyrene(0.14 to 1.11 μmol g −1 ) or phenanthrene (0.08 to 0.62 μmol g −1 )‐dosed sediments for month‐long exposures. Phenanthrene was only slightly toxic with 12±3% mortality at the highest sediment dose (0.62 μmol g −1 ). Failure to attain and maintain toxic residue body burdens, based on a nonpolar narcosis concentration of approximately 6 μmol g −1 , accounts for the low mortality. Phenanthrene toxicokinetic parameters were essentially constant among all doses and consistent with previous measures. Sediment concentration was a poor representation of dose for mortality by pyrene. The relative pyrene distribution among the <63‐μm particles increased in the smallest‐sized particles at larger doses. An apparent stimulation of pyrene accumulation was observed as a peak in uptake clearance values between sediment concentrations of 0.16 and 0.26 μmol g −1 dry sediment. (Uptake clearance is the amount of source scavenged of contaminant per mass of organism per time.) The pyrene particle‐size distribution and the variation in kinetics with dose provide a partial explanation for the poor representation of dose by the sediment concentration. The pyrene body burdens provided a good dose response yielding LD50 values of 6.3 (4.6–41.7, 95% C.I.) and 9.4 (7.9–54.2) μmol g −1 for two experiments. These values are consistent with the residue concentrations for 50% mortality by a nonpolar narcosis mechanism. Comparing the experimental and predicted equilibrium partitioning‐based sediment concentrations for 50% mortality, the equilibrium prediction overestimates the toxic pyrene sediment concentration by approximately a factor of ten. Diporeia behavior, differential particle‐size distribution, and kinetic limitations appear as likely reasons for the variation between calculated and observed concentrations required to produce mortality.