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Biochemical and mutagenic characterization of plant‐activated aromatic amines
Author(s) -
Plewa Michael J.,
Gichner Tomáš,
Xin Hua,
Seo KwangYoung,
Smith Shan R.,
Wagner Elizabeth D.
Publication year - 1993
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620120805
Subject(s) - plant cell , yeast , peroxidase , bacteria , biochemistry , plant system , biology , chemistry , pollutant , microbiology and biotechnology , enzyme , genetics , gene , organic chemistry
Plant activation is the process by which promutagenic agents are activated into rautagens by plant systems. With the widespread use of agricultural chemicals on crop plants and with the global exposure of plants to pollutants, the possibility exists that plant‐activated agents may be introduced into the human food chain. The plant cell/microbe coincubation assay uses cultured plant cell suspensions as the activating system and bacteria or yeast cells as the genetic indicator organism. After a treatment time, the microbes are plated on selective medium. In this way the activation system and the genetic system can be independently studied. In addition, the viability of the plant cells and the microbial cells can be independently determined so that the toxicity of a test agent can be evaluated. Using cytochrome P450 monooxygenase and peroxidase inhibitors, we are studying the biochemical mechanisms of plant activation of environmental contaminants, especially aromatic amines. We propose a working model for the activation of aromatic amines by cultured tobacco cells into stable frameshift mutagens.