Premium
Effect of wastewater from olive oil mills on nitrogenase activity and growth of Azotobacter chroococcum
Author(s) -
GarcíaBarrionuevo A.,
Moreno E.,
QuevedoSarmiento J.,
GonzálezLópez J.,
RamosCormenzana A.
Publication year - 1993
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620120205
Subject(s) - azotobacter chroococcum , azotobacter , chemistry , nitrogenase , wastewater , olive oil , food science , carbon source , botany , nitrogen fixation , horticulture , biochemistry , bacteria , biology , nitrogen , organic chemistry , environmental engineering , environmental science , genetics , inoculation
The effect of 1, 5, 10, 15, and 20% (v/v) of wastewater from olive oil mills (“alpechin”) was investigated in cells of Azotobacter chroococcum grown in chemically defined media (N‐free or with NH + 4 ) and dialyzed‐soil media. Wastewater from olive oil mills at concentrations of 1 to 20% (v/v) significantly reduced dinitrogen fixation and growth of Azotobacter chroococcum in chemically defined N‐free medium, whereas the presence of 1 to 15% had a stimulatory effect on dinitrogen fixation and growth of Azotobacter in dialyzed‐soil medium. Azotobacter chroococcum grew on NH + 4 media (without glucose) amended with alpechin, suggesting that these wastes were utilized by Azotobacter as a carbon source.