Effect of wastewater from olive oil mills on nitrogenase activity and growth of  Azotobacter chroococcum | Zendy

GarcíaBarrionuevo A. | Zendy; Moreno E. | Zendy; QuevedoSarmiento J. | Zendy; GonzálezLópez J. | Zendy; RamosCormenzana A. | Zendy

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Effect of wastewater from olive oil mills on nitrogenase activity and growth of Azotobacter chroococcum

Author(s) -

GarcíaBarrionuevo A.,

Moreno E.,

QuevedoSarmiento J.,

GonzálezLópez J.,

RamosCormenzana A.

Publication year - 1993

Publication title -

environmental toxicology and chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.1

H-Index - 171

eISSN - 1552-8618

pISSN - 0730-7268

DOI - 10.1002/etc.5620120205

Subject(s) - azotobacter chroococcum , azotobacter , chemistry , nitrogenase , wastewater , olive oil , food science , carbon source , botany , nitrogen fixation , horticulture , biochemistry , bacteria , biology , nitrogen , organic chemistry , environmental engineering , environmental science , genetics , inoculation

The effect of 1, 5, 10, 15, and 20% (v/v) of wastewater from olive oil mills (“alpechin”) was investigated in cells of Azotobacter chroococcum grown in chemically defined media (N‐free or with NH + 4 ) and dialyzed‐soil media. Wastewater from olive oil mills at concentrations of 1 to 20% (v/v) significantly reduced dinitrogen fixation and growth of Azotobacter chroococcum in chemically defined N‐free medium, whereas the presence of 1 to 15% had a stimulatory effect on dinitrogen fixation and growth of Azotobacter in dialyzed‐soil medium. Azotobacter chroococcum grew on NH + 4 media (without glucose) amended with alpechin, suggesting that these wastes were utilized by Azotobacter as a carbon source.

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