Premium
Uptake and elimination of radioactivity from rainbow trout ( oncorhynchus mykiss ) following exposure to [ 14 C‐ring]‐2‐phenyldodecane
Author(s) -
Burke Alison B.,
Millburn Peter,
Huckle Keith R.,
Hutson David H.
Publication year - 1991
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620101112
Subject(s) - bioconcentration , rainbow trout , chemistry , partition coefficient , bioaccumulation , toxicokinetics , clearance , fish <actinopterygii> , trout , environmental chemistry , biotransformation , metabolism , zoology , radiochemistry , chromatography , biology , fishery , biochemistry , medicine , urology , enzyme
The bioconcentration and elimination of radioactivity was monitored in rainbow trout exposed to [ring‐ 14 C]‐2‐phenyldodecane in a flow‐through system. The uptake of radioactivity from the water at 15°C into the tissues was rapid, with equilibrium concentrations being approached at about 10 d. The bioconcentration factor (BCF) ( C fish / C water ) measured in whole fish was 443. After 28 d exposure, fish were transferred to tanks containing clean water, and the clearance of radioactivity from tissues was monitored for a further 28 d in two test groups; one was maintained on a growth diet and the other on a rationed feed. Radioactivity was cleared rapidly from fish in both groups. Bile was a significant route of elimination. The effect of temperature was studied. When fish were exposed at a water temperature of 4°C, less [ 14 C]‐2‐phenyldodecane was taken up, giving a lower BCF value for radioactivity (149) than the level at 15°C. The higher BCF at 15°C was due to the greater effect of temperature on uptake ( k 1 values) than on the elimination ( k 2 values). The BCF value predicted for 2‐phenyldodecane based on water solubility or partition coefficient data is between 20,000 and 3,500,000. However, 2‐phenyldodecane is susceptible to rapid and extensive biotransformation by fish. This metabolism limits the bioconcentration of the detergent alkylate in fish tissues.