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Genotoxicologic evaluation of ozonated/chlorinated drinking water: Cytogenetic effects of XAD‐fractions on cultured human cells
Author(s) -
Varga Csaba
Publication year - 1991
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620100807
Subject(s) - clastogen , sister chromatid exchange , chemistry , water treatment , chromatography , lymphocyte , toxicity , filtration (mathematics) , environmental chemistry , carcinogen , nuclear chemistry , biochemistry , genotoxicity , in vitro , biology , organic chemistry , environmental engineering , immunology , statistics , mathematics , engineering
Ozonated and chlorinated drinking water produced by a surface water treatment plant was examined to determine if genotoxins were formed by the treatment process The examined technology included ozonation (oxidation) and chlorination (disinfection), but excluded granular activated carbon or slow sand filtration following the ozonation step Investigations involved cytogenetic assays, chromosomal aberration, and sister chromatid exchange (SCE) analyses of 70‐h human peripheral lymphocyte cultures treated with water isolates obtained on XAD resins Neutral XAD 2/4, XAD‐8, and acidic XAD‐2/4–8 fractions were tested separately in 1‐, 10‐, 20‐μl/ml concentrations of the 7,000‐fold isolates without metabolic activation A weak but significant ( p < 0.05) SCE‐inducing activity could be detected only upon treatment with the neutral XAD‐2/4 fraction Clastogenicity and mitotic inhibition were not observed in the given concentration range