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In vitro cytotoxicity of chlorophenols to goldfish GF‐scale (GFS) cells and quantitative structure‐activity relationships
Author(s) -
Saito Hotaka,
Sudo Masato,
Shigeoka Tadayoshi,
Yamauchi Fumio
Publication year - 1991
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620100212
Subject(s) - cytotoxicity , partition coefficient , chemistry , ecotoxicity , toxicity , environmental chemistry , in vitro , quantitative structure–activity relationship , linear regression , stereochemistry , biochemistry , chromatography , organic chemistry , machine learning , computer science
Abstract GF‐Scale (GFS) cells, a fibroblastic cell line derived from the scale of goldfish, were used for the determination of the cytotoxicity of chlorophenols (CPs) and the quantitative structure‐activity relationships (QSAR) studies. As the cytotoxicity end point, the amount of neutral red retained by viable cells after exposure to chemicals was quantified. The sequence of cytotoxicity based on the concentration of chemicals that reduced uptake of neutral red by 50% (NR50) was penta‐Cl (PCP) > 2,4,5‐Cl 3 > 2,3,4‐Cl 3 > 2,3,4,6‐Cl 4 > 3,5‐Cl 2 > 3,4‐Cl 2 > 2,4‐Cl 2 > 2,5‐Cl 2 > 2,3‐Cl 2 > 2,4,6‐Cl 3 > 3‐Cl > 4‐Cl > 2,6‐Cl > 2‐Cl > phenol. The in vitro cytotoxicity of these chemicals was found to be significantly correlated to their in vivo acute toxicity to aquatic species, and NR50 values were correlated with six physicochemical parameters of chlorophenols. Log P ow ( n ‐octanol/water partition coefficient) gave the best correlation in simple linear regression analysis, as is frequently stated in toxicity studies with aquatic animals. Multiparametric linear regression equations yielded improved correlation coefficients and predictive capabilities, including the log P ow and pK a . These results suggest that in vitro fish cytotoxicity assays using the GFS cell lines are useful for ecotoxicity screening of aquatic pollutants.