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Biodehalogenation: Oxidative and reductive metabolism of 1,1,2‐trichloroethane by Pseudomonas Putida —Biogeneration of vinyl chloride
Author(s) -
Castro C.E.,
Belser N.O.
Publication year - 1990
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620090603
Subject(s) - pseudomonas putida , chemistry , vinyl chloride , reductive dechlorination , chloroacetic acid , biochemistry , biotransformation , metabolism , camphor , oxidative phosphorylation , enzyme , trichloroethylene , organic chemistry , medicinal chemistry , biodegradation , copolymer , polymer
Resting cell suspensions of the camphor‐grown soil organism Pseudomonas putida PpG‐786, ATCC culture 29607, dehalogenate 1,1,2‐trichloroethane by two pathways under aerobic conditions. The dominant pathway (85%) is oxidative ( k ox , 4 × 10 −19 L/org/sec) and leads in sequence to chloroacetic acid and glyoxylic acid. A competitive reductive pathway (15%) ( k red , 0.7 × 10 −19 L/org/sec) occurs simultaneously and yields vinyl chloride exclusively. Glucose‐grown cells are ineffective in these reactions, and carbon monoxide completely inhibits these transformations. These findings implicate cytochrome P‐450 cam as the responsible enzyme in vivo. Overall rates of conversion of 1,1,2‐trichloroethane by the organism and the isolated enzyme correspond to those obtained previously with ethylene dibromide. The results suggest that reductive microbial dehalogenation can occur in the environment even under aerobic conditions.