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Reductive dechlorination of 4‐chlororesorcinol by anaerobic microorganisms
Author(s) -
Boyd Stephen A.,
Fathepure Babu Z.,
Tiedje James M.
Publication year - 1987
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620061204
Subject(s) - reductive dechlorination , anaerobic exercise , chemistry , biodegradation , yeast , microorganism , detoxication , environmental chemistry , substrate (aquarium) , resorcinol , biotransformation , bacteria , biology , biochemistry , organic chemistry , ecology , physiology , genetics , enzyme
The anaerobic dechlorination of 4‐chlororesorcinol (4‐Cl‐Res) to resorcinol (1,3‐dihydroxybenzene) by fresh anaerobic digester sludge and by an enriched microbial culture was studied. Complete dechlorination of 50 ppm 4‐Cl‐Res in fresh sludge required about four weeks and occurred without a lag. An anaerobic community capable of degrading 4‐Cl‐Res was enriched with 4‐Cl‐Res as the substrate. Dechlorination of 4‐Cl‐Res by this enrichment occurred at a relatively low rate (0.3 μmol mg −1 d −1 ) and after a long lag period (three weeks). Addition of yeast extract or trypticase markedly enhanced the rate of dechlorination. The rate increased from 0.3 μmol mg −1 d −1 in the unamended enrichment to 1.1 to 2.6 μmol mg −1 d −1 in cultures amended with 0.1% yeast extract or 0.1% trypticase. Also, the lag period was reduced from approximately three weeks to less than 2 d. The ability to stimulate reductive dechlorination is important because it demonstrates that this process can be enhanced by carbon additions that may lead to practical schemes for fostering detoxication mechanisms in nature.