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Detection of cadmium‐binding proteins in fish chronically exposed to heavy metals
Author(s) -
Benson William H.,
Birge Wesley J.
Publication year - 1987
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620060806
Subject(s) - cadmium , metallothionein , hatchery , fractionation , environmental chemistry , fish <actinopterygii> , cytosol , chemistry , biology , biochemistry , fishery , chromatography , enzyme , organic chemistry
Studies were undertaken to determine the presence of cadmium‐binding proteins in fish from a metal‐contaminated flyash disposal pond and relatively uncontaminated hatchery ponds. Fractionation of gill cytosolic proteins by gel filtration indicated three classes of cadmium‐binding proteins. While no cadmium was detected in the metallothionein (MT)‐like fraction of the hatchery fish, 1% of the cytosolic cadmium was detected in this fraction for ash pond fish. Cadmium concentrations in the flyash pond were previously reported as ranging from 0.30 to 0.98 μg/L. This level of exposure may be insufficient to induce significant biosynthesis of MT‐like proteins.