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Chronic effects of DI‐2‐ethylhexyl phthalate on biochemical composition, survival and reproduction of Daphnia magna
Author(s) -
Knowles Charles O.,
McKee Michael J.,
Palawski Donald U.
Publication year - 1987
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620060305
Subject(s) - daphnia magna , phthalate , toxicant , reproduction , biology , daphnia , fecundity , glycogen , rna , offspring , dna , toxicity , toxicology , zoology , biochemistry , chemistry , ecology , gene , genetics , population , pregnancy , demography , sociology , organic chemistry , crustacean
Daphnia magna Straus were exposed for 21 d to 0, 12, 27, 72, 158 and 811 μg/L di‐2‐ethylhexyl phthalate (DEHP). On days 7 and 21 of exposure, levels of protein, RNA, DNA, glycogen and total lipid were determined and related to effects on survival and reproduction. The maximum allowable toxicant concentration based on survival and reproduction was between 158 and 811 μg/L DEHP. However, survival and reproduction were not the most sensitive parameters measured. A no observed effect concentration of 72 μg/L was identified by DNA content per individual on day 7, by RNA/DNA ratio on day 7 and by surfacing behavior of Daphnia on day 0. Reduced protein growth was associated with increased RNA/DNA and protein/RNA/DNA ratios. Glycogen was the only biomolecule affected in Daphnia exposed to 811 μg/L DEHP for 21 d.