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Improved sea urchin DNA‐based embryo growth toxicity test
Author(s) -
Jackim Eugene,
Nacci Diane
Publication year - 1986
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620050608
Subject(s) - sea urchin , toxicity , embryo , biology , dna , zoology , fishery , chemistry , microbiology and biotechnology , genetics , organic chemistry
This article presents an improved DNA‐based embryo toxicity test in which growth is measured as the increase in DNA during a 5‐h postfertilization period. The test, using direct fluoro‐metric determination of DNA rather than thymidine incorporation, is fast, sensitive, easy to conduct and correlates well with other 48‐ and 96‐h acute aquatic tests. The need for radioisotopes has been eliminated and data variability is substantially reduced. It is recommended that this test be incorporated into a battery of rapid tests used to estimate aquatic toxicity.