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Inter‐ and intralaboratory variability in Daphnia magna effluent toxicity test results
Author(s) -
Grothe Donald R.,
Kimerle Richard A.
Publication year - 1985
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620040208
Subject(s) - daphnia magna , effluent , coefficient of variation , ec50 , toxicity , cladocera , bioassay , daphnia , round robin test , ecotoxicology , standard deviation , toxicology , biology , environmental chemistry , zoology , environmental science , chromatography , chemistry , ecology , mathematics , environmental engineering , statistics , crustacean , in vitro , biochemistry , organic chemistry
A nine‐laboratory round‐robin study of the Daphnia magna static, acute, effluent toxicity test was conducted to examine inter‐ and intralaboratory variability in test results. A single effluent sample was split and sent to three government, three commercial and three industrial aquatic laboratories. Each laboratory followed a specifically designed test protocol and reported the number of daphnids immobilized in each of seven effluent test concentrations at 24 h and 48 h of exposure. The mean 48‐h EC50 value for all data was 5.3% effluent. The range was 3.5 to 9.1% effluent, so there is a factor of 2.6 between the highest and lowest EC50 values. The pooled within‐laboratory standard deviation and coefficient of variation were 0.91 and 16%, respectively. Combined inter‐and intralaboratory estimates of standard deviation and coefficient of variation were 1.8 and 33%, respectively. The results of this study show that Daphnia magna effluent toxicity data can be reproduced both within and between laboratories when clearly defined test protocols are employed.