Evaluation and development of static shake‐flask biodegradation test systems | Zendy

Blum James E. | Zendy; Stevenson Cathy A. | Zendy; Stainken Dennis M. | Zendy

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Evaluation and development of static shake‐flask biodegradation test systems

Author(s) -

Blum James E.,

Stevenson Cathy A.,

Stainken Dennis M.

Publication year - 1983

Publication title -

environmental toxicology and chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.1

H-Index - 171

eISSN - 1552-8618

pISSN - 0730-7268

DOI - 10.1002/etc.5620020202

Subject(s) - laboratory flask , biodegradation , incubation , volume (thermodynamics) , chemistry , incubation period , chromatography , biochemistry , organic chemistry , physics , quantum mechanics

Several biodegradation flask systems were evaluated to assess the effects of flask shape and volume and length of incubation on the recovery of 14 CO 2 . During a 6‐h incubation period, it was demonstrated that the recovery of 14 CO 2 decreased as the volume of the flask (50 ml to 2 L) and the length of the diffusion path increased. Increasing the incubation time improved the performance of flasks, which were relatively inefficient at 6 h. Selected flask systems were further assessed, measuring the extent of CO 2 evolution from nonradiolabeled linear alkylate sulfonate and glucose by a mixed inoculum.

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