Premium
Indigenous Fish–Based Assessment of Genotoxic Potentials of the Helong Reservoir in Guangzhou, China
Author(s) -
Xian Hongyi,
Tang Meilin,
Chen Ying,
Lian Zhenwei,
Li Yanfeng,
Peng Xiaowu,
Hu Dalin
Publication year - 2021
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5043
Subject(s) - oreochromis , labeo , micronucleus test , water quality , comet assay , micronucleus , tilapia , toxicology , biology , veterinary medicine , environmental science , fishery , fish <actinopterygii> , chemistry , ecology , dna damage , toxicity , dna , medicine , genetics , organic chemistry
The present study was conducted to assess the genotoxic potential of water from the Helong Reservoir, which was designated as a strategic drinking water source by the Guangdong Provincial Government of China in October 2016. Four kinds of common indigenous fish samples ( Labeo rohita , Cirrhinus molitorella , red tilapia, and Oreochromis niloticus ) were collected at 6 sampling sites during the period from July to November 2020. Fish from the clean drinking water source of the upper reaches of the Liuxihe Reservoir in Guangzhou were collected as the control. Both the alkaline single cell gel electrophoresis assay and the micronucleus test were used to detect DNA damage and the micronucleus rate in erythrocytes of fish samples, respectively. The results indicated that there was a significant increase in comet tail length, Olive tail moment, and micronucleus rates of all fish samples compared with those of the control ( p < 0.05). The order of sensitivity to DNA damage and micronucleus formation was Labeo rohita > Cirrhinus molitorella > red tilapia > Oreochromis niloticus . The results of the 2 kinds of experiments were in perfect agreement with each other. We conclude that there are obvious genotoxic effects from the water in the Helong Reservoir. As a strategic drinking water source, the safety of the Reservoir water quality should be considered. The local government should put the restoration of the Helong Reservoir water quality on the agenda as soon as possible. Environ Toxicol Chem 2021;40:1917–1925. © 2021 SETAC