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Prioritization of 10 organic flame retardants using an avian hepatocyte toxicogenomic assay
Author(s) -
PagéLarivière Florence,
Chiu Suzanne,
Jones Stephanie P.,
Farhat Amani,
Crump Doug,
O'Brien Jason M.
Publication year - 2018
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.4260
Subject(s) - toxicogenomics , toxicity , cormorant , transcriptome , environmental toxicology , chemistry , in vitro toxicology , in vitro , environmental chemistry , biology , biochemistry , gene , ecology , gene expression , organic chemistry , predation
As the number of chemicals developed and used by industry increases, the inherent limitations of traditional toxicology approaches become an unavoidable issue. To help meet the demand for toxicity evaluation, new methods, such as high‐throughput toxicity screening, are currently being developed to permit rapid determination of toxic, molecular, and/or biochemical effects of a wide range of chemicals. In the present study, we demonstrate the utility of an avian in vitro toxicogenomics screening approach to determine the cytotoxic and transcriptomic effects of 10 organic flame retardants (OFRs) currently of international priority for ecological risk evaluation to prioritize and inform future toxicological studies. Hepatocytes from 2 avian species, chicken and double‐crested cormorant, were prepared and exposed for 24 h to various concentrations (0–300 μM) of the following 10 OFRs: Chemical Abstracts Service registration numbers 29761‐21‐5, 56803‐37‐3 ( p ‐tert‐butylphenyl diphenyl phosphate [BPDP]), 65652‐41‐7, 68937‐41‐7 (phenol, isopropylated, phosphate [3:1] [IPPP]), 95906‐11‐9, 19186‐97‐1, 26040‐51‐7, 35948‐25‐5, 21850‐44‐2, and 25713‐60‐4. Cell viability, the 7‐ethoxyresorufin‐ O ‐deethylase assay, and transcriptomic analysis using species‐specific ToxChip polymerase chain reaction arrays were performed to evaluate the in vitro effect of these OFRs. Of the 10 OFRs assessed, BPDP and IPPP elicited the strongest cytotoxic and transcriptomic responses in both chicken and double‐crested cormorant hepatocytes and are therefore recommended as priority candidates for further wildlife toxicological investigations. Environ Toxicol Chem 2018;37:3134–3144. © 2018 Crown in the right of Canada. Published by Wiley Periodicals Inc. on behalf of SETAC.