Premium
Recovery from reproductive and morphological abnormalities in medaka ( Oryzias latipes ) following a 14‐day exposure to diclofenac
Author(s) -
Yokota Hirofumi,
Higashi Kaho,
Hanada Erina,
Matsuzaki Ei,
Tsuruda Yukinari,
Suzuki Tomoko,
Nakano Eiko,
Eguchi Sayaka
Publication year - 2017
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.3899
Subject(s) - oryzias , diclofenac , biology , fecundity , andrology , vitellogenin , physiology , fish <actinopterygii> , anatomy , zoology , medicine , pharmacology , fishery , population , environmental health
Abstract Mating pairs of medaka ( Oryzias latipes ) were exposed to diclofenac at measured concentrations of 0 (control), 7.1, 37, and 78 μg/L for 14 d under static‐renewal conditions. Effects on reproductive success, as well as morphological abnormalities, of the fish were assessed. During the exposure period, both fecundity and fertility were significantly decreased in the 37‐ and 78‐μg/L treatment groups, and swollen abdomens in females were observed in all exposure groups. Notably, a defect of the lower jaw was also observed in 4 male fish: 2 at 37 μg/L and 2 at 78 μg/L of diclofenac. Subsequently, we investigated whether the reproductive and morphological abnormalities induced by diclofenac would be permanent or reversible once the medaka were returned to clean water. The reproductive ability of paired medaka was gradually restored to fish that were cultured in clean water for 14 d. After this period in clean water, we also observed a noticeable decrease in swollen abdomens in females; however, mandibular defects in the males remained, even after the 14‐d recovery period. Radiographic and histochemical examinations revealed that diclofenac might affect bone remodeling in the lower jaw of male medaka because of a disruption in osteoclast function. These results suggest that reproductive impairments in pairs of medaka exposed to diclofenac may be reversible but that skeletal deformities (i.e., mandibular defect) in males may be persistent. Environ Toxicol Chem 2017;36:3277–3283. © 2017 SETAC