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Pathways of root uptake and membrane transport of Cd 2+ in the zinc/cadmium hyperaccumulating plant Sedum plumbizincicola
Author(s) -
Li LianZhen,
Tu Chen,
Wu LongHua,
Peijnenburg Willie J.G.M.,
Ebbs Stephen,
Luo YongMing
Publication year - 2017
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.3625
Subject(s) - hyperaccumulator , chemistry , cadmium , channel blocker , ion transporter , biochemistry , membrane , environmental chemistry , phytoremediation , calcium , heavy metals , organic chemistry
Uptake and membrane transport of cadmium (Cd) in roots of the hyperaccumulator Sedum plumbizincicola X.H. Guo et S.B. Zhou ex L.H. Wu was characterized by assessing the impact of various inhibitors and ion channel blockers on Cd accumulation as well as the real‐time net Cd 2+ flux at the roots with application of the scanning ion‐selective electrode technique. The uncouplers 2,4‐dinitrophenol and P‐type adenosine triphosphatase inhibitor Na 3 VO 4 significantly limited Cd 2+ uptake and transport kinetics in the root of S. plumbizincicola . These findings indicate that Cd is actively taken up into the roots. The Cd content in plant was significantly decreased with pretreatments of the Ca 2+ channel blocker La 3+ or Gd 3+ and the K + channel blocker tetraethylammonium, as well as in the presence of higher concentration of Ca 2+ and K + . These findings indicated that uptake of Cd 2+ into the root of S. plumbizincicola proceeds through ion channels that are permeable to both Ca 2+ and K + as confirmed by the direct evidence of real‐time net Cd 2+ fluxes at the root surface in the treatments with ion channel inhibitors, as well as in the presence of elevated concentrations of Ca 2+ and K + . In addition, the results suggested a role for phytochelatin and protein synthesis in mediating Cd 2+ uptake by S. plumbizincicola . These findings increase the understanding of Cd 2+ uptake and membrane transport pathways in roots of the Zn/Cd hyperaccumulator S. plumbizincicola . Environ Toxicol Chem 2017;36:1038–1046. © 2016 SETAC