Premium
Using an interlaboratory study to revise methods for conducting 10‐d to 42‐d water or sediment toxicity tests with Hyalella azteca
Author(s) -
Ivey Chris D.,
Ingersoll Chris G.,
Brumbaugh William G.,
Hammer Edward J.,
Mount Dave R.,
Hockett J. Russell,
NorbergKing Teresa J.,
Soucek Dave,
Taylor Lisa
Publication year - 2016
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.3417
Subject(s) - hyalella azteca , sediment , environmental chemistry , reproduction , toxicity , aquatic toxicology , toxicology , biology , environmental science , chemistry , ecology , amphipoda , organic chemistry , crustacean , paleontology
Abstract Studies have been conducted to refine US Environmental Protection Agency, ASTM International, and Environment Canada standard methods for conducting 42‐d reproduction tests with Hyalella azteca in water or in sediment. Modifications to the H. azteca method include better‐defined ionic composition requirements for exposure water (i.e., >15 mg/L of chloride and >0.02 mg/L of bromide) and improved survival, growth, and reproduction with alternate diets provided as increased rations over time in water‐only or whole‐sediment toxicity tests. A total of 24 laboratories volunteered to participate in the present interlaboratory study evaluating the performance of H. azteca in 42‐d studies in control sand or control sediment using the refined methods. Improved growth and reproduction of H. azteca was observed with 2 alternate diets of 1) ramped diatoms ( Thalassiosira weissflogii ) + ramped Tetramin or 2) yeast–cerophyll–trout chow (YCT) + ramped Tetramin, especially when compared with results from the traditional diet of 1.8 mg YCT/d. Laboratories were able to meet proposed test acceptability criteria and in most cases had lower variation in growth or reproduction compared with previous interlaboratory studies using the traditional YCT diet. Laboratory success in conducting 42‐d H. azteca exposures benefited from adherence to several key requirements of the detailed testing, culturing, and handling methods. Results from the present interlaboratory study are being used to help revise standard methods for conducting 10‐d to 42‐d water or sediment toxicity exposures with H. azteca . Environ Toxicol Chem 2016;35:2439–2447. © 2016 SETAC