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Juvenile zebrafish in the vitellogenin blank period as an alternative test organism for evaluation of estrogenic activity of chemicals
Author(s) -
Wang Jun,
Zhao Fei,
Shan Ruihou,
Tian Hua,
Wang Wei,
Ru Shaoguo
Publication year - 2016
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.3328
Subject(s) - vitellogenin , organism , period (music) , juvenile , zebrafish , yolk , biology , bioassay , toxicology , fish <actinopterygii> , ecology , biochemistry , fishery , physics , acoustics , gene , paleontology
The present study aimed to determine the suitable development period for zebrafish to evaluate estrogenic activities accurately. An enzyme‐linked immunosorbent assay was developed and used to detect the vitellogenin (Vtg)‐derived yolk proteins and newly produced Vtg, and 9 d to 56 d posthatching was determined as the Vtg‐blank period. Juveniles in this period were found to have lower baseline Vtg levels than adult males and were considered an alternative test organism for detecting environmental estrogens. Environ Toxicol Chem 2016;35:1783–1787. © 2015 SETAC