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Morphological and transcriptional responses of Lycopersicon esculentum to hexavalent chromium in agricultural soil
Author(s) -
Li ShiGuo,
Hou Jing,
Liu XinHui,
Cui BaoShan,
Bai JunHong
Publication year - 2016
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.3315
Subject(s) - lycopersicon , hexavalent chromium , chemistry , chromium , ecotoxicology , environmental chemistry , biology , horticulture , organic chemistry
Abstract The carcinogenic, teratogenic, and mutagenic effects of hexavalent chromium (Cr[VI]) on living organisms through the food chain raise the immediate need to assess the potential toxicological impacts of Cr(VI) on human health. Therefore, the concentration‐dependent responses of 12 Cr(VI)‐responsive genes selected from a high‐throughput Lycopersicon esculentum complementary DNA microarray were examined at different Cr concentrations. The results indicated that most of the genes were differentially expressed from 0.1 mg Cr/kg soil, whereas the lowest‐observable‐adverse‐effect concentrations of Cr(VI) were 1.6 mg Cr/kg soil, 6.4 mg Cr/kg soil, 3.2 mg Cr/kg soil, and 0.4 mg Cr/kg soil for seed germination, root elongation, root biomass, and root morphology, respectively, implying that the transcriptional method was more sensitive than the traditional method in detecting Cr(VI) toxicity. Dose‐dependent responses were observed for the relative expression of expansin ( p = 0.778), probable chalcone‐flavonone isomerase 3 ( p = –0.496), and 12S seed storage protein CRD ( p = –0.614); therefore, the authors propose the 3 genes as putative biomarkers in Cr(VI)‐contaminated soil. Environ Toxicol Chem 2016;35:1751–1758. © 2015 SETAC