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Examining impacts of current‐use pesticides in Southern Ontario using in situ exposures of the amphipod Hyalella azteca
Author(s) -
Bartlett Adrienne J.,
Struger John,
Grapentine Lee C.,
Palace Vince P.
Publication year - 2016
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.3265
Subject(s) - hyalella azteca , amphipoda , pesticide , environmental science , current (fluid) , ecology , environmental chemistry , biology , oceanography , crustacean , chemistry , geology
In situ exposures with Hyalella azteca were used to assess impacts of current‐use pesticides in Southern Ontario, Canada. Exposures were conducted over 2 growing seasons within areas of high pesticide use: 1 site on Prudhomme Creek and 3 sites on Twenty Mile Creek. Three sites on Spencer Creek, an area of low pesticide use, were added in the second season. Surface water samples were collected every 2 wk to 3 wk and analyzed for a suite of pesticides. Hyalella were exposed in situ for 1 wk every 4 wk to 6 wk, and survival and acetylcholinesterase (AChE) activity were measured. Pesticides in surface waters reflected seasonal use patterns: lower concentrations in spring and fall and higher concentrations during summer months. Organophosphate insecticides (chlorpyrifos, azinphos methyl, diazinon) and acid herbicides (2,4‐dichlorophenoxyacetic acid [2,4‐D], mecoprop) were routinely detected in Prudhomme Creek, whereas neutral herbicides (atrazine, metolachlor) dominated the pesticide signature of Twenty Mile Creek. Spencer Creek contained fewer pesticides, which were measured at lower concentrations. In situ effects also followed seasonal patterns: higher survival and AChE activity in spring and fall, and lower survival and AChE activity during summer months. The highest toxicity was observed at Prudhomme Creek and was primarily associated with organophosphates. The present study demonstrated that current‐use pesticides in Southern Ontario were linked to in situ effects and identified sites of concern requiring further investigation. Environ Toxicol Chem 2016;35:1224–1238. © 2015 Crown in the Right of Canada.