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Maternal repeated oral exposure to microcystin‐LR affects neurobehaviors in developing rats
Author(s) -
Li XiaoBo,
Zhang Xin,
Ju Jingjuan,
Li Yunhui,
Yin Lihong,
Pu Yuepu
Publication year - 2015
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.2765
Subject(s) - malondialdehyde , offspring , morris water navigation task , neurotoxicity , microcystin lr , open field , superoxide dismutase , righting reflex , lipid peroxidation , hippocampus , biology , toxicity , medicine , endocrinology , oxidative stress , physiology , reflex , pregnancy , cyanobacteria , bacteria , genetics
Abstract Microcystins are toxic peptides secreted by certain water blooms of toxic cyanobacteria. The most widely studied microcystin is microcystin‐LR (MC‐LR), which exhibits hepatotoxicity and neurotoxicity. However, limited information is available regarding the effects on offspring following maternal exposure. The present study was conducted to observe the effects of progestational exposure to MC‐LR on postnatal development in rats. Female Sprague‐Dawley rats (28 d old) were randomly divided into a control group and 3 treatment groups (1.0 µg MC‐LR/kg body wt, 5.0 µg MC‐LR/kg body wt, and 20.0 µg MC‐LR/kg body wt), with 7 rats per group. The MC‐LR was administered through gavage once every 48 h for 8 wk. Pure water was used as control. Each female rat was mated with an unexposed adult male rat. Motor development, behavioral development, and learning ability of pups were detected using surface righting reflex, negative geotaxis, and cliff avoidance tests on postnatal day 7. Open‐field and Morris water maze tests were performed on postnatal day 28 and day 60. The levels of lipid peroxidation products and antioxidant indices in the rat hippocampus were also detected. Pups from the MC‐LR‐treated groups had significantly lower scores than controls in the cliff avoidance test ( p < 0.05). Cognitive impairment, malondialdehyde level, and total superoxide dismutase activity significantly increased in MC‐LR–exposed pups compared with controls ( p < 0.05). Therefore, the present study reveals that maternal exposure to MC‐LR has adverse effects on neurodevelopment in rat offspring. Environ Toxicol Chem 2015;34:64–69. © 2014 SETAC