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A simple method to determine mineralization of 14 C‐labeled compounds in soil
Author(s) -
Myung Kyung,
Madary Michael W.,
Satchivi Norbert M.
Publication year - 2014
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.2558
Subject(s) - mineralization (soil science) , chemistry , pellets , environmental chemistry , lime , soil test , mineralogy , soil water , environmental science , soil science , organic chemistry , geology , nitrogen , paleontology , oceanography
Abstract Degradation of organic compounds in soil is often determined by measuring the decrease of the parent compound and analyzing the occurrence of its metabolites. However, determining carbon species as end products of parent compound dissipation requires using labeled materials that allow more accurate determination of the environmental fate of the compound of interest. The current conventional closed system widely used to monitor degradation of 14 C‐labeled compounds in soil is complex and expensive and requires a specialized apparatus and facility. In the present study, the authors describe a simple system that facilitates measurement of mineralization of 14 C‐labeled compounds applied to soil samples. In the system, soda lime pellets to trap mineralized 14 C‐carbon species, including carbon dioxide, were placed in a cup, which was then inserted above the treated soil sample in a tube. Mineralization of [ 14 C]2,4‐D applied to soil samples in the simple system was compared with that in the conventional system. The simple system provided an equivalent detection of 14 C‐carbon species mineralized from the parent compound. The results demonstrate that this cost‐ and space‐effective simple system is suitable for examining degradation and mineralization of 14 C‐labeled compounds in soil and could potentially be used to investigate their mineralization in other biological matrices. Environ Toxicol Chem 2014;33:1303–1307. © 2014 SETAC