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Inter‐laboratory trial of a standardized sediment contact test with the aquatic plant Myriophyllum aquaticum (ISO 16191)
Author(s) -
Feiler Ute,
Ratte Monika,
Arts Gertie,
Bazin Christine,
Brauer Frank,
Casado Carmen,
Dören Laszlo,
Eklund Britta,
Gilberg Daniel,
Grote Matthias,
Gonsior Guido,
Hafner Christoph,
Kopf Willi,
Lemnitzer Bernd,
Liedtke Anja,
Matthias Uwe,
Okos Ewa,
Pandard Pascal,
Scheerbaum Dirk,
SchmittJansen Mechthild,
Stewart Kathleen,
Teodorovic Ivana,
Wenzel Andrea,
Pluta HansJürgen
Publication year - 2014
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.2483
Subject(s) - bioassay , sediment , environmental science , toxicology , environmental chemistry , chemistry , biology , ecology , paleontology
Abstract A whole‐sediment toxicity test with Myriophyllum aquaticum has been developed by the German Federal Institute of Hydrology and standardized within the International Organization for Standardization (ISO; ISO 16191). An international ring‐test was performed to evaluate the precision of the test method. Four sediments (artificial, natural) were tested. Test duration was 10 d, and test endpoint was inhibition of growth rate ( r ) based on fresh weight data. Eighteen of 21 laboratories met the validity criterion of r ≥ 0.09 d −1 in the control. Results from 4 tests that did not conform to test‐performance criteria were excluded from statistical evaluation. The inter‐laboratory variability of growth rates (20.6%–25.0%) and inhibition (26.6%–39.9%) was comparable with the variability of other standardized bioassays. The mean test‐internal variability of the controls was low (7% [control], 9.7% [solvent control]), yielding a high discriminatory power of the given test design (median minimum detectable differences [MDD] 13% to 15%). To ensure these MDDs, an additional validity criterion of CV ≤ 15% of the growth rate in the controls was recommended. As a positive control, 90 mg 3,5‐dichlorophenol/kg sediment dry mass was tested. The range of the expected growth inhibition was proposed to be 35 ± 15%. The ring test results demonstrated the reliability of the ISO 16191 toxicity test and its suitability as a tool to assess the toxicity of sediment and dredged material. Environ Toxicol Chem 2014;33:662–670. © 2013 SETAC