Optimizing fluorescent protein choice for transgenic embryonic medaka models | Zendy

Loire Nicolas | Zendy; Barbeau Emilie | Zendy; Lemkine Gregory F. | Zendy; Léonard Marc A. | Zendy; Tindall Andrew J. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Optimizing fluorescent protein choice for transgenic embryonic medaka models

Author(s) -

Loire Nicolas,

Barbeau Emilie,

Lemkine Gregory F.,

Léonard Marc A.,

Tindall Andrew J.

Publication year - 2013

Publication title -

environmental toxicology and chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.1

H-Index - 171

eISSN - 1552-8618

pISSN - 0730-7268

DOI - 10.1002/etc.2324

Subject(s) - fluorescence , autofluorescence , oryzias , transgene , fluorescent protein , embryonic stem cell , embryo , excitation wavelength , genetically modified mouse , biology , biophysics , chemistry , green fluorescent protein , microbiology and biotechnology , fish <actinopterygii> , biochemistry , optics , physics , gene , fishery

Early‐life‐stage transgenic medaka are recognized as a pertinent model by the Organisation for Economic Co‐operation and Development and are noncompliant with the European definition of a laboratory animal. However, autofluorescence confounds readout of fluorescent biomarkers. The authors determined the fluorescence emission spectrum of different embryonic stages of medaka submitted to a range of excitation wavelengths. This allows selection of high signal‐to‐noise ratio fluorescent proteins and combining multiple biomarkers within a single embryo. Environ Toxicol Chem 2013;32:2396–2401. © 2013 SETAC

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research