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Optimizing fluorescent protein choice for transgenic embryonic medaka models
Author(s) -
Loire Nicolas,
Barbeau Emilie,
Lemkine Gregory F.,
Léonard Marc A.,
Tindall Andrew J.
Publication year - 2013
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.2324
Subject(s) - fluorescence , autofluorescence , oryzias , transgene , fluorescent protein , embryonic stem cell , embryo , excitation wavelength , genetically modified mouse , biology , biophysics , chemistry , green fluorescent protein , microbiology and biotechnology , fish <actinopterygii> , biochemistry , optics , physics , gene , fishery
Early‐life‐stage transgenic medaka are recognized as a pertinent model by the Organisation for Economic Co‐operation and Development and are noncompliant with the European definition of a laboratory animal. However, autofluorescence confounds readout of fluorescent biomarkers. The authors determined the fluorescence emission spectrum of different embryonic stages of medaka submitted to a range of excitation wavelengths. This allows selection of high signal‐to‐noise ratio fluorescent proteins and combining multiple biomarkers within a single embryo. Environ Toxicol Chem 2013;32:2396–2401. © 2013 SETAC