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Development of an in vitro thin‐film solid‐phase microextraction method to determine the bioavailability of xenoestrogens in soil
Author(s) -
Engler Katherine Neafsey,
Lemley Ann T.
Publication year - 2013
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.2292
Subject(s) - bioavailability , biosolids , environmental chemistry , eisenia fetida , solid phase microextraction , triclosan , xenoestrogen , chemistry , diffusive gradients in thin films , phthalate , chromatography , environmental science , environmental engineering , biology , organic chemistry , toxicity , pharmacology , gas chromatography–mass spectrometry , medicine , mass spectrometry , genetics , pathology , estrogen receptor , cancer , breast cancer
Biosolids applied to agricultural fields, parks, and other areas represent significant sources of estrogen‐like endocrine disrupting compound (EEDC) inputs to soil. It is important to determine the bioavailability of EEDCs in soil to inform risk assessment concerning their environmental presence; Eisenia fetida (earthworms) are typically used in traditional in vivo bioavailability experiments. The development of an in vitro bioavailability method will decrease time, expense, and use of solvents in future analyses. A thin‐film solid‐phase microextraction (TF‐SPME) method for determining the bioavailability of several EEDCs detected in biosolids was developed and optimized. It was found that the TF‐SPME method could be used to calculate equilibrium porewater concentrations of diethylhexyl phthalate, bisphenol A, benzophenone, and triclosan at environmentally relevant concentrations in artificial soil within 88 min. The potential and limitations of using TF‐SPME–generated porewater concentrations to predict E. fetida tissue concentrations are discussed. Environ Toxicol Chem 2013;32:1962–1968. © 2013 SETAC