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A practical quantification method for Heinz bodies in birds applicable to rapid response field scenarios
Author(s) -
Fallon Jesse A.,
Hopkins William A.,
Fox Lee
Publication year - 2013
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.2058
Subject(s) - field (mathematics) , heinz body , environmental science , biochemical engineering , biology , engineering , mathematics , hemoglobin , biochemistry , pure mathematics
Oil‐induced oxidative injury to red blood cells results in Heinz body hemolytic anemia. Here, we evaluated three Heinz body staining techniques in brown pelican ( Pelecanus occidentalis ) blood. Using a range of in vitro acetylphenylhydrazine incubations, we validated a field‐adapted technique against laboratory wet‐mounts and verified the stability of this technique for one month following preparation. Employing this technique during petrochemical spill responses allows for delays between sample collection and analysis. Environ. Toxicol. Chem. 2013;32:401–405. © 2012 SETAC