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Physiological effect of anatase TiO 2 nanoparticles on Lemna minor
Author(s) -
Song Guanling,
Gao Yuan,
Wu Hao,
Hou Wenhua,
Zhang Chunyang,
Ma Huiquan
Publication year - 2012
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.1933
Subject(s) - lemna minor , catalase , chemistry , superoxide dismutase , titanium dioxide , phytotoxicity , environmental chemistry , nuclear chemistry , reactive oxygen species , zeta potential , aquatic plant , botany , antioxidant , nanoparticle , biochemistry , chemical engineering , biology , materials science , nanotechnology , macrophyte , ecology , engineering
Manufactured metal oxide nanoparticles (NPs) are being used on a large scale, and these particles will inevitably reach a body of water through wastewater and urban runoff. The ecotoxicological study of these NPs on hydrophyte is limited at present. Lemna minor was exposed to media with different concentrations of titanium dioxide (TiO 2 ) NPs or bulk TiO 2 for 7 d. The changes in plant growth, chlorophyll, antioxidant defense enzymes (peroxidase [POD], catalase [CAT], and superoxide dismutase [SOD] activities), and malondialdehyde (MDA) content were measured in the present study. The particle size of TiO 2 NPs and the zeta potential of TiO 2 NPs and of bulk TiO 2 in the culture media were also analyzed to complementally study the toxicity of these materials on duckweed. The results showed that the effect of TiO 2 NPs on plant growth was more obvious than bulk TiO 2. Titanium dioxide NPs stimulated plant growth in low concentrations, but inhibited plant growth at high concentrations. The POD, SOD, and CAT activity of Lemna minor increased when TiO 2 NP concentration was lower than 200 mg/L to eliminate accumulated reactive oxygen species in plant cells. The SOD activity decreased when the TiO 2 NP concentration was higher than 200 mg/L, and the plant cell membrane encountered serious damage from 500 mg/L TiO 2 NP concentration in the culture media. Environ. Toxicol. Chem. 2012; 31: 2147–2152. © 2012 SETAC