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Effects of chronic copper exposure on development and survival in the southern leopard frog ( Lithobates [Rana] sphenocephalus )
Author(s) -
Lance Stacey L.,
Erickson Matthew R.,
Flynn R. Wesley,
Mills Gary L.,
Tuberville Tracey D.,
Scott David E.
Publication year - 2012
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.1849
Subject(s) - leopard frog , lithobates , larva , amphibian , metamorphosis , biology , hatching , leopard , embryo , zoology , ecology , tadpole (physics) , fishery , physics , particle physics
Exposure to environmental contaminants contributes to the global decline of amphibian populations. The impacts of organic contaminants on amphibians are well documented. However, substantially less is known concerning the potential effects of metals on amphibian populations. Copper (Cu) is an essential element, but it can be toxic at concentrations only slightly higher than the normal physiological range. The present study examines the effects of chronic Cu exposure on embryos and larvae of southern leopard frogs, Lithobates (Rana) sphenocephalus . Groups of eggs from multiple clutches were collected from two wetlands and exposed to a range of Cu concentrations (0–150 µg/L) until they reached the free‐swimming stage, and then individual larvae were reared to metamorphosis. Higher Cu concentrations significantly reduced embryo survival to the free‐swimming stage but did not further reduce survival to metamorphosis. Larval period was affected by Cu treatment, but the clutch from which larvae originated (i.e., parentage) explained a higher proportion of the variation. Embryo survival to hatching varied significantly among clutches, ranging from 42.9 to 79.2%. Measurable levels of Cu were found in larvae with body burdens up to 595 µg Cu/g dry mass in the 100 µg/L treatment, and larval Cu body burdens were higher than in metamorphs. The present study also demonstrated that higher initial egg density ameliorated embryo mortality at higher Cu levels and should be accounted for in future studies. Environ. Toxicol. Chem. 2012; 31: 1587–1594. © 2012 SETAC