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Quick assessment of cytotoxins effect on Daphnia magna using in vivo fluorescence microscopy
Author(s) -
Teplova Vera V.,
AndreevaKovalevskaya Zhanna I.,
Sineva Elena V.,
Solonin Alexander S.
Publication year - 2010
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.169
Subject(s) - daphnia magna , toxicity , fluorescence , fluorescence microscope , in vivo , environmental chemistry , chemistry , toxicology , biology , microbiology and biotechnology , optics , physics , organic chemistry
A novel approach to contaminant toxicity screening is proposed. The use of fluorescent microscopy with fluorescent dyes allows for assessing intoxication of Daphnia magna tissues, at various stages of exposure, to contaminants present in water. As shown, D. magna may not only be used as a test species in toxicity tests based on its lethality, but due to its translucency and application of fluorescent probes, separate steps of its intoxication and dying can be visualized. Using a variety of fluorescent probes, the present study also contributes to a better understanding of the toxicity mechanisms. Environ. Toxicol. Chem. 2010;29:1345–1348. © 2010 SETAC